Aim: COVID‑19 pandemic response measures adversely affected the psychological effects of health‑care professionals due to disruption of daily life, sense of uncertainty, fear of getting sick, and the perception of working in a dangerous environment. In this study, we assessed the level of depression and anxiety symptoms in health‑care professionals who had interaction with COVID‑19 patients both before and after vaccination. Materials and Methods: The participants in this prospective cohort study, which took place between July 24, 2020 and April 30, 2021, were 233 health‑care workers who were employed in the hospital’s COVID area. Participants were divided into two groups as pre‑COVID‑19 vaccine group (Group 1; n = 98) and postvaccine group (Group 2; n = 135), both groups received the Hospital Anxiety and Depression Scale. Results: The mean score of the Group 1 anxiety subscale was 15.64 ± 2.112, and the mean score of the depression subscale was 15.19 ± 1.762. The same scores were 9.65 ± 5.535 and 9.13 ± 4.984, respectively, in Group 2. There was a statistically significant difference between the groups (P = 0.001). Conclusion: In our research, we have seen that the application of the vaccine has positive effects on the psychological state of health workers who are directly exposed to COVID‑19 patients. We think that the therapies or preventive measures that are developed during the pandemic phase will lessen the possibility of sadness and anxiety in health‑care personnel and boost the effectiveness of the effort to combat the disease.